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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on <t>YPD</t> or <t>YPD</t> <t>plates</t> with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.
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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on <t>YPD</t> or <t>YPD</t> <t>plates</t> with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.
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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on <t>YPD</t> or <t>YPD</t> <t>plates</t> with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.
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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on <t>YPD</t> or <t>YPD</t> <t>plates</t> with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.
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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on <t>YPD</t> or <t>YPD</t> <t>plates</t> with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.
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Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on YPD or YPD plates with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.

Journal: mSphere

Article Title: Loss-of-Function ROX1 Mutations Suppress the Fluconazole Susceptibility of upc2A Δ Mutation in Candida glabrata , Implicating Additional Positive Regulators of Ergosterol Biosynthesis

doi: 10.1128/msphere.00830-21

Figure Lengend Snippet: Deletion of ROX1 suppresses fluconazole and hypoxia hypersusceptibility of upc2A Δ mutants. (A) Tenfold dilution series of the indicated strains were spotted on YPD or YPD plates with the indicated concentrations of fluconazole and incubated for 24 or 48 h at 30°C. (B) The indicated strains were plated on YPD and incubated at 30°C in ambient air or in a GasPak to establish a low-oxygen environment as described in Materials and Methods.

Article Snippet: Overnight cultures were spun down and plated on YPD plates supplemented with 100 μg/ml of nourseothricin (NAT) (Jena Bioscience, Jena, Germany).

Techniques: Incubation